HOME > Publications of Department of Stem Cell Biology and Histology 2020〜2011

Publications of Department of Stem Cell Biology and Histology


  • Fujita Y, Komatsu M, San Eun Lee, Kushida Y, Nakayama-Nishimura C, Matsumura W, Takashima S, Shinkuma S, Nomura T, Masutomi N, Kawamura M, Dezawa M, Shimizu H. Intravenous injection of Muse cells as a potential therapeutic approach for epidermolysis bullosa. Journal of Investigative Dermatology 2020 inpress
  • Sato T, Wakao S, Kushida Y, Tatsumi K, Kitada M, Abe T, Niizuma K, Tominaga T, Kushimoto S, Dezawa M. A Novel Type of Stem Cells Double-Positive for SSEA-3 and CD45 in Human Peripheral Blood. Cell Transplantation 2020 inpress
  • Abe T, Aburakawa D, Niizuma K, Iwabuchi N, Kajitani T, Wakao S, Kushida Y, Dezawa M, Borlongan CV, Tominaga T. Intravenously Transplanted Human Multilineage-Differentiating Stress-Enduring Cells Afford Brain Repair in a Mouse Lacunar Stroke Model. Stroke. 2020 Feb;51(2):601-611. doi: 10.1161/STROKEAHA.119.026589. Epub 2019 Dec 12.
  • Ozuru R, Wakao S, Tsuji T, Ohara N, Matsuba T, Amuran MY, Isobe J, Iino M, Nishida N, Matsumoto S, Iwadate K, Konishi N, Yasuda K, Tashiro K, Hida M, Yadoiwa A, Kato S, Yamashita E, Matsumoto S, Kurozawa Y, Dezawa M, Fujii J. Rescue from Stx2-Producing E. coli-Associated Encephalopathy by Intravenous Injection of Muse Cells in NOD-SCID Mice. Mol Ther. 2020 Jan 8;28(1):100-118. doi: 10.1016/j.ymthe.2019.09.023. Epub 2019 Oct 1.


  • Dezawa M, Niizuma K, Tominaga T. [Actualization of Neural Regenerative Medicine by Intravenous Drip of Donor-derived Muse Cells]. Brain Nerve. 2019 Aug;71(8):895-900. doi: 10.11477/mf.1416201372. Review. Japanese.
  • Tezuka Y, Yamazaki Y, Kitada M, Morimoto R, Kudo M, Seiji K, Takase K, Kawasaki Y, Mitsuzuka K, Ito A, Nishikawa J, Asai N, Nakamura Y, Gomez-Sanchez CE, Ito S, Dezawa M, Sasano H, Satoh F. 18-Oxocortisol Synthesis in Aldosterone-Producing Adrenocortical Adenoma and Significance of KCNJ5 Mutation Status. Hypertension. 2019 Jun;73(6):1283-1290. doi: 10.1161/HYPERTENSIONAHA.118.12064.
  • Leng Z, Sun D, Huang Z, Tadmori I, Chiang N, Kethidi N, Sabra A, Kushida Y, Fu YS, Dezawa M, He X, Young W. Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting. Cell Transplant. 2019 Jul;28(7):907-923. doi: 10.1177/0963689719844260. Epub 2019 Apr 18.
  • Dezawa M. Expanding Investigations Into Endogenous Reparative Pluripotent Muse Cells Through Clinical Application, Based on the Intravenous Drip of Donor-derived Muse Cells. Impact, vol.2019, no.3, Mar 2019, pp. 38-40, doi: https://doi.org/10.21820/23987073.2019.3.38.
  • Kitada M, Murakami T, Wakao S, Li G, Dezawa M. Direct conversion of adult human skin fibroblasts into functional Schwann cells that achieve robust recovery of the severed peripheral nerve in rats. Glia. 2019 May;67(5):950-966. doi: 10.1002/glia.23582. Epub 2019 Jan 13.


  • Dezawa M. Clinical Trials of Muse Cells. Adv Exp Med Biol. 2018;1103:305-307. doi: 10.1007/978-4-431-56847-6_17.
  • Tatsumi K, Kushida Y, Wakao S, Kuroda Y, Dezawa M. Protocols for Isolation and Evaluation of Muse Cells. Adv Exp Med Biol. 2018;1103:69-101. doi: 10.1007/978-4-431-56847-6_4.
  • Kushida Y, Wakao S, Dezawa M. Muse Cells Are Endogenous Reparative Stem Cells. Adv Exp Med Biol. 2018;1103:43-68. doi: 10.1007/978-4-431-56847-6_3.
  • Wakao S, Kushida Y, Dezawa M. Basic Characteristics of Muse Cells. Adv Exp Med Biol. 2018;1103:13-41. doi: 10.1007/978-4-431-56847-6_2.
  • Dezawa M. The Muse Cell Discovery, Thanks to Wine and Science. Adv Exp Med Biol. 2018;1103:1-11. doi: 10.1007/978-4-431-56847-6_1.
  • Kitada M, Wakao S, Dezawa M. Intracellular signaling similarity reveals neural stem cell-like properties of ependymal cells in the adult rat spinal cord. Dev Growth Differ. 2018 Aug;60(6):326-340. doi: 10.1111/dgd.12546. Epub 2018 Jul 9.
  • Yabuki H, Wakao S, Kushida Y, Dezawa M, Okada Y. Human Multilineage-differentiating Stress-Enduring Cells Exert Pleiotropic Effects to Ameliorate Acute Lung Ischemia-Reperfusion Injury in a Rat Model. Cell Transplant. 2018 Jun;27(6):979-993. doi: 10.1177/0963689718761657. Epub 2018 Apr 30.
  • Amin M, Kushida Y, Wakao S, Kitada M, Tatsumi K, Dezawa M. Cardiotrophic Growth Factor-Driven Induction of Human Muse Cells Into Cardiomyocyte-Like Phenotype. Cell Transplant. 2018 Feb;27(2):285-298. doi: 10.1177/0963689717721514.
  • Houkin K, Shichinohe H, Abe K, Arato T, Dezawa M, Honmou O, Horie N, Katayama Y, Kudo K, Kuroda S, Matsuyama T, Miyai I, Nagata I, Niizuma K, Sakushima K, Sasaki M, Sato N, Sawanobori K, Suda S, Taguchi A, Tominaga T, Yamamoto H, Yamashita T, Yoshimine T; Working Group for Guidelines on Development of Cell-Based Products for the Treatment of Cerebral Infarction. Accelerating Cell Therapy for Stroke in Japan: Regulatory Framework and Guidelines on Development of Cell-Based Products. Stroke. 2018 Apr;49(4):e145-e152. doi: 10.1161/STROKEAHA.117.019216. Epub 2018 Mar 16. Review. No abstract available.
  • Hosoyama K, Wakao S, Kushida Y, Ogura F, Maeda K, Adachi O, Kawamoto S, Dezawa M, Saiki Y. Intravenously injected human multilineage-differentiating stress-enduring cells selectively engraft into mouse aortic aneurysms and attenuate dilatation by differentiating into multiple cell types. J Thorac Cardiovasc Surg. 2018 Jun;155(6):2301-2313.e4. doi: 10.1016/j.jtcvs.2018.01.098. Epub 2018 Feb 21.
  • Yamada Y, Wakao S, Kushida Y, Minatoguchi S, Mikami A, Higashi K, Baba S, Shigemoto T, Kuroda Y, Kanamori H, Amin M, Kawasaki M, Nishigaki K, Taoka M, Isobe T, Muramatsu C, Dezawa M, Minatoguchi S. S1P-S1PR2 Axis Mediates Homing of Muse Cells Into Damaged Heart for Long-Lasting Tissue Repair and Functional Recovery After Acute Myocardial Infarction. Circ Res. 2018 Apr 13;122(8):1069-1083. doi: 10.1161/CIRCRESAHA.117.311648. Epub 2018 Feb 23.
  • Tanaka T, Nishigaki K, Minatoguchi S, Nawa T, Yamada Y, Kanamori H, Mikami A, Ushikoshi H, Kawasaki M, Dezawa M, Minatoguchi S. Mobilized Muse Cells After Acute Myocardial Infarction Predict Cardiac Function and Remodeling in the Chronic Phase. Circ J. 2018 Jan 25;82(2):561-571. doi: 10.1253/circj.CJ-17-0552. Epub 2017 Sep 20.


  • Mahmoud EE, Kamei N, Shimizu R, Wakao S, Dezawa M, Adachi N, Ochi M. Therapeutic Potential of Multilineage-Differentiating Stress-Enduring Cells for Osteochondral Repair in a Rat Model. Stem Cells Int. 2017;2017:8154569. doi: 10.1155/2017/8154569. Epub 2017 Oct 29.
  • Suzuki JI, Dezawa M, Kitada M. Prolonged but non-permanent expression of a transgene in ependymal cells of adult rats using an adenovirus-mediated transposon gene transfer system. Brain Res. 2017 Nov 15;1675:20-27. doi: 10.1016/j.brainres.2017.08.033. Epub 2017 Sep 1.
  • Yamasaki T, Wakao S, Kawaji H, Koizumi S, Sameshima T, Dezawa M, Namba H. Genetically Engineered Multilineage-Differentiating Stress-Enduring Cells as Cellular Vehicles against Malignant Gliomas. Mol Ther Oncolytics. 2017. Jun 15;6:45-56. doi: 10.1016/j.omto.2017.06.001. eCollection 2017 Sep 15.
  • Uchida N, Kushida Y, Kitada M, Wakao S, Kumagai N, Kuroda Y, Kondo Y, Hirohara Y, Kure S, Chazenbalk G, Dezawa M. Beneficial Effects of Systemically Administered Human Muse Cells in Adriamycin Nephropathy. J Am Soc Nephrol 2017. Oct;28(10):2946-2960. doi: 10.1681/ASN.2016070775. Epub 2017 Jul 3.
  • Iseki M, Kushida Y, Wakao S, Akimoto T, Mizuma M, Motoi F, Asada R, Shimizu S, Unno M, Chazenbalk G, Dezawa M. Human Muse Cells, Nontumorigenic Pluripotent-Like Stem Cells, Have Liver Regeneration Capacity Through Specific Homing and Cell Replacement in a Mouse Model of Liver Fibrosis. Cell Transplantation, vol. 26, pp. 821-840, 2017, doi:10.3727/096368916X693662
  • Alessio N, Ozcan S, Tatsumi K, Murat A, Peluso G, Dezawa M, Galderisi U. The secretome of MUSE cells contains factors that may play a role in regulation of stemness, apoptosis and immunomodulation. Cell Cycle 2017 vol.16, no.1, 33-44, doi:10.1080/15384101.2016.1211215
  • Uchida H, Niizuma K, Kushida Y, Wakao S, Tominaga T, Borlongan CV, Dezawa M. Human Muse Cells Reconstruct Neuronal Circuitry in Subacute Lacunar Stroke Model. Stroke. 2017, 48(2):428-435. doi:10.1161/STROKEAHA.116.014950. Epub 2016 Dec 20.
  • Shimamura N, Kakuta K, Wang L, Naraoka M, Uchida H, Wakao S, Dezawa M, Ohkuma H. Neuro-regeneration therapy using human Muse cells is highly effective in a mouse intracerebral hemorrhage model. Exp Brain Res. 2017 235(2):565-572. doi:10.1007/s00221-016-4818-y. Epub 2016 Nov 5.


  • Kitada M, Takeda K, Dezawa M. Regulation of DM-20 mRNA expression and intracellular translocation of glutathione-S-transferase pi isoform during oligodendrocyte differentiation in the adult rat spinal cord. Histochem Cell Biol (2016) 146:45-57, doi:10.1007/s00418-016-1421-z
  • Dezawa M. Muse cells provide the pluripotency of mesenchymal stem cells: direct contribution of Muse cells to tissue regeneration. Cell Transplantation, vol.25, no.5, 2016, 849-861(13), doi:10.3727/096368916X690881
  • Katagiri H, Kushida Y, Nojima M, Kuroda Y, Wakao S, Ishida K, Endo F, Kume K, Takahara T, Nitta H, Tsuda H, Dezawa M, Nishizuka SS. A Distinct Subpopulation of Bone Marrow Mesenchymal Stem Cells, Muse Cells, Directly Commit to the Replacement of Liver Components. Am J Transplant. 2016 Feb;16(2):468-83. doi: 10.1111/ajt.13537. Epub 2015 Dec 11.
  • Nakamura Y, Kitada M, Satoh F, Maekawa T, Morimoto R, Yamazaki Y, Ise K, Gomez-Sanchez CE, Ito S, Arai Y, Dezawa M, Sasano H. Intratumoral heterogeneity of steroidogenesis in aldosterone-producing adenoma revealed by intensive double- and triple-immunostaining for CYP11B2/B1 and CYP17. Mol Cell Endocrinol. vol. 422, 15 Feb. 2016, 57-63, doi: 10.1016/j.mce.2015.11.014.
  • Takeda K, Dezawa M, Kitada M. The expression of PLP/DM-20 mRNA is restricted to the oligodendrocyte-lineage cells in the adult rat spinal cord. Histochem Cell Biol (2016) 145:147-161, doi: 10.1007/s00418-015-1384-5.


  • Uchida H, Sakata H, Fujimura M, Niizuma K, Kushida Y, Dezawa M, Tominaga T. Experimental model of small subcortical infarcts in mice with long-lasting functional disabilities. Brain Res. 2015 Dec 10;1629:318-28. doi: 10.1016/j.brainres.2015.10.039. Epub 2015 Oct 29.
  • Uchida H, Morita T, Niizuma K, Kushida Y, Kuroda Y, Wakao S, Sakata H, Matsuzaka Y, Mushiake H, Tominaga T, Borlongan CV, Dezawa M. Transplantation of Unique Subpopulation of Fibroblasts, Muse Cells, Ameliorates Experimental Stroke Possibly via Robust Neuronal Differentiation. Stem Cells. 2016 Jan;34(1):160-73. doi: 10.1002/stem.2206. Epub 2015 Sep 28.
  • Hiwatashi N, Hirano S, Suzuki R, Kawai Y, Mizuta M, Kishimoto Y, Tateya I, Kanemaru SI, Nakamura T, Dezawa M, Ito J. Comparison of ASCs and BMSCs combined with atelocollagen for vocal fold scar regeneration. Laryngoscope. Vol.126, Issue 5, May 2016, 1143-1150 doi: 10.1002/lary.25667.
  • Wakao S, Dezawa M. Current status and future prospects of Muse cell research. Nihon Yakurigaku Zasshi. 2015;145(6):299-305. doi: 10.1254/fpj.145.299.
  • Endo F, Komine O, Fujimori-Tonou N, Katsuno M, Jin S, Watanabe S, Sobue G, Dezawa M, Wyss-Coray T, Yamanaka K. Astrocyte-derived TGF-β1 accelerates disease progression in ALS mice by interfering with the neuroprotective functions of microglia and T cells. Cell Rep. 2015 Apr 28;11(4):592-604, doi:10.1016/j.celrep.2015.03.053
  • Yamauchi T, Kuroda Y, Morita T, Shichinohe H, Houkin K, Dezawa M, Kuroda S. Therapeutic effects of human multilineage-differentiating stress enduring (MUSE) cell transplantation into infarct brain of mice. PLoS One. 2015 Mar 6;10(3):e0116009. doi: 10.1371/journal.pone.0116009. eCollection 2015.


  • Wakao S, Matsuse D, Dezawa M. Mesenchymal stem cells as a source of Schwann cells: their anticipated use in peripheral nerve regeneration. Cells Tissues Organs. 2014;200(1):31-41.
  • Mannoji C, Koda M, Kamiya K, Dezawa M, Hashimoto M, Furuya T, Okawa A, Takahashi K, Yamazaki M. Transplantation of human bone marrow stromal cell-derived neuroregenrative cells promotes functional recovery after spinal cord injury in mice. Acta Neurobiol Exp (Wars). 2014;74(4):479-88.
  • Wakao S, Akashi H, Dezawa M. Muse cells, a novel type of non-tumorigenic pluripotent stem cells, that reside in human mesenchymal tissues. Spinal Surgery 28; p17-23, 2014
  • Okano T, Dezawa M. Anat. Rec (Hoboken). A new age of regenerative medicine: fusion of tissue engineering and stem cell research. 297(1):4-5, 2014.
  • Albertine KH, Dezawa M. Anat. Rec (Hoboken). A new age of regenerative medicine: fusion of tissue engineering and stem cell research. 297(1):1-3, 2014.
  • Wakao S, Akashi H, Kushida Y, Dezawa M. Muse cells, a novel type of non-tumorigenic pluripotent stem cells, reside in human mesenchymal tissues. Pathology International. 64(1):1-9, 2014.
  • Y. Kuroda, M. Dezawa. Mesenchymal stem cells and their subpopulation, pluripotent Muse cells, in basic research and regenerative medicine. Anat Rec. 297(1):98-110, 2014.
  • Ogura F, Wakao S, Kuroda Y, Tsuchiyama K, Bagheri M, Heneidi S, Chazenbalk G, Aiba S, Dezawa M. Human adipose tissue possesses a unique population of pluripotent stem cells with non-tumorigenic and low telomerase activities: potential implications in regenerative medicine. Stem Cells Dev. 23(7):717-28, 2014.


  • Ishikawa H, Tajiri N, Shinozuka K, Vasconcellos J, Kaneko Y, Lee HJ, Mimura O, Dezawa M, Kim SU, Borlongan CV. Vasculogenesis in Experimental Stroke After Human Cerebral Endothelial Cell Transplantation. Stroke. 44(12):3473-81, 2013.
  • Ishikawa H, Tajiri N, Vasconcellos J, Kaneko Y, Mimura O, Dezawa M, Borlongan CV. Ischemic Stroke Brain Sends Indirect Cell Death Signals to the Heart. Stroke. 44(11):3175-82, 2013.
  • Kanemaru SI, Kitani Y, Ohno S, Shigemoto T, Kojima T, Ishikawa S, Mizuta M, Hirano S, Nakamura T, Dezawa M. Functional regeneration of laryngeal muscle using bone marrow-derived stromal cells. Laryngoscope. 123(11):2728-34, 2013.
  • Furuya T, Hashimoto M, Koda M, Murata A, Okawa A, Dezawa M, Matsuse D, Tabata Y, Takahashi K, Yamazaki M. Treatment with basic fibroblast growth factor-incorporated gelatin hydrogel does not exacerbate mechanical allodynia after spinal cord contusion injury in rats. J Spinal Cord Med. 36(2):134-9, 2013.
  • Kuroda Y, Wakao S, Kitada M, Murakami T, Nojima M, and Dezawa M. Isolation, culture and evaluation of Multilineage-differentiating Stress Enduring (Muse) cells. Nature Protocols. 8(7):1391-415, 2013.
  • Shigemoto T, Kuroda Y, Wakao S, Dezawa M. A novel approach to collect satellite cells from adult skeletal muscles based on their stress tolerance. STEM CELLS Translational Medicine. 2(7):488-98, 2013.
  • Tsuchiyama K, Wakao S, Kuroda Y, Ogura F, Nojima M, Sawaya N, Yamazaki K, Aiba S, Dezawa M. Functional melanocytes are readily reprogrammable from multilineage-differentiating stress-enduring (Muse) cells, distinct stem cells in human fibroblasts. J Invest. Dermatol. 133(10):2425-35, 2013.
  • Hayashi T, Wakao S, Kitada M, Ose T, Watabe H, Kuroda Y, Mitsunaga K, Matsuse D, Shigemoto T, Ito A, Ikeda H, Fukuyama H, Onoe H, Tabata Y, Dezawa M. Autologous engraftment of A9 dopaminergic neurons induced from mesenchymal stem cells in parkinsonian rhesus macaques. J. Clin. Invest. 123(1):272-84, 2013.
  • Aizawa-Kohama M, Endo T, Kitada M, Wakao S, Sumiyoshi A, Matsuse D, Kuroda Y, Morita T, Riera JJ, Kawashima R, Tominaga T, Dezawa M. Transplantation of bone marrow stromal cell-derived neural precursor cells ameliorates deficits in a rat model of complete spinal cord transection. Cell Transplant. 22(9):1613-25, 2013.
  • Wakao S, Kitada M, Dezawa M. The elite and stochastic model for iPS cell generation: Multilineage- differentiating stress enduring (Muse) cells are readily reprogrammable into iPS cells. Cytometry A. 83(1):18-26, 2013.


  • Wakao S, Kitada M, Kuroda Y, Ogura F, Murakami T, Niwa A, Dezawa M. Morphologic and gene expression criteria for identifying human induced pluripotent stem cells. PLoS One. 2012;7(12):e48677. doi: 10.1371/journal.pone.0048677.
  • Wakao S, Kuroda Y, Ogura F, Shigemoto T, Dezawa M.  Regenerative Effects of Mesenchymal Stem Cells: Contribution of Muse Cells, a Novel Pluripotent Stem Cell Type that Resides in Mesenchymal Cells. Cells. 1: 1045-60, 2012.
  • Kitada M, Dezawa M. Parkinson's disease and mesenchymal stem cells:potential for cell-based therapy, Parkinson's Disease. 2012:873706. doi: 10.1155/2012/873706, 2012.
  • Kitada M, Wakao S, Dezawa M. Muse cells and induced pluripotent stem cell: implication of the elite model. Cell Mol Life Sci. 69(22):3739-50, 2012.
  • Wakao S, Kitada M, Kuroda Y, Dezawa M. Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages. Liver Stem Cells: Methods and Protocols, Methods in Molecular Biology, Springer Protocols. vol. 826: 89-102, 2012.


  • Kuroda Y, Kitada M, Wakao S, Dezawa M. Mesenchymal stem cells and umbilical cord as sources for Schwann cell differentiation: their potential in peripheral nerve repair. The Open Tissue Engineering and Regenerative medicine Journal. 4:54-63, 2011.
  • Kuroda Y, Kitada M, Wakao S, Dezawa M. Bone Marrow Mesenchymal Cells: How Do They Contribute to Tissue Repair and Are They Really Stem Cells? Arch. Immunol. Ther. Exp. 59:369-78, 2011.
  • Wakao S, Kitada M, Kuroda Y, Shigemoto T, Matsuse D, Akashi H, Tanimura Y, Tsuchiyama K, Kikuchi T, Goda M, Nakahata T, Fujiyoshi Y, Dezawa M. Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 108(24):9875-80, 2011.
  • Matsuse D, Kitada M, Ogura F, Wakao S, Kohama M, Kira JI, Tabata Y, Dezawa M.Combined transplantation of bone marrow stromal cell-derived neural progenitor cells with a collagen sponge and fibroblast growth factor (bFGF) releasing microspheres enhances recovery after cerebral ischemia in rats. Tissue Eng. Part A. 17(15-16):1993-2004, 2011.
  • Kitada M, Kuroda Y, Dezawa M. Lectins as a tool for detecting neural stem/progenitor cells in the adult mouse brain. Anat. Rec. 294(2):305-21, 2011.
  • Kamada T, Koda M, Dezawa M, Anahara R, Toyama Y, Yoshinaga K, Hashimoto M, Koshizuka S, Nishio Y, Mannoji C, Okawa A, Yamazaki M. Transplantation of human bone marrow stromal cell-derived Schwann cells reduces cystic cavity and promotes functional recovery after contusion injury of adult rat spinal cord. Neuropathology. 31(1):48-58, 2011.